HiDef® B8 Stem Cell Growth Medium Compliance:Research

HiDef® B8 Stem Cell Growth Medium Compliance:ResearchDefined Biosciences HiDef B8 is a complete, serum free, defined formulation designed for the feeder free maintenance and expansion of human pluripotent stem cells (PSCs) in an undifferentiated state. HiDef B8 is provided in two formats to offer flexibility for use: The HiDef B8 complete medium kit, including the 400X Supplement vial and our DMEM F12 basal medium; The HiDef B8 400X Supplement only (basal medium NOT included), for the modern convenience

offering a comprehensive buffering system which helps to maintain pH stability and minimize the effect of CO2 fluctuations in standard incubator conditions for mammalian cell culture

undifferentiated stem cell culture

particularly in serum-free workflows

4 µg cm-2 (e

The HiDef® B8 complete medium kit

DMEM/F12 is a 1:1 mixture of DMEM (a modified Basal Medium Eagle (BME) formulation with higher concentrations of amino acids and vitamins) and Ham’s F-12 (modified from Ham’s F-10 with increased choline

and primary cell culture

HiDef-ITS should be used with other growth factors

HiDef N-2 is a combination of insulin (to stimulate glucose uptake)

sodium selenite (a potent antioxidant and common medium component)

and progesterone (for promoting growth and neuronal differentiation and stimulation)

with fewer medium changes